# THM-ESATT Nanomaterial Immunotoxicology & Safe-by-Design Desk
> Independent preclinical nanomedicine, 2D nanomaterial immunotoxicology, and Safe-by-Design (SSbD) biomaterial evidence portal (`https://thm-esatt.net/`).

## Editorial Scope & Independence
- Focus: Standardized preclinical assay cascades (NCI-NCL, EU-NCL, OECD WPMN, ISO 10993-22), 2D graphene/MXene single-cell immune profiling, orthogonal endotoxin interference controls (chromogenic LAL vs. recombinant Factor C vs. Monocyte Activation / TNF-alpha inhibitor controls under ISO 29701), CARPA complement screening (NCL ITA-5.1 SC5b-9 / C3a), and biocompatible biosensing scaffolds.
- Provenance: Independent scientific portal. Not affiliated with, endorsed by, or a legal continuation of the European Space Agency (ESA), ESA Topical Team THM-ESATT (2018-2021), WHISKIES, WHISPER, University of Bremen ComNets, or Delogu Lab. Historical consortium context is archived inside `/about/#archive-esa-tt-mission`.

## Primary Canonical Routes
- `/` — Two-Rail SOP Monograph & Quick Triage (4-Material Assay Selector, Embedded Endotoxin/CARPA Triage, 4-Standard Cascade Matrix)
- `/endotoxin-carpa-workbench/` — Full Nanomaterial Endotoxin Interference, CARPA Risk & BET Surface-Area Dose Workbench
- `/assay-cascade/` — Cross-Framework Assay Cascade Comparison Matrix (NCI-NCL, EU-NCL, Graphene Flagship 3-Descriptor, ISO 10993-22)
- `/graphene-2d-immunotoxicity/` — 2D Graphene, GO/rGO & MXene Single-Cell Immunotoxicity & 3-Descriptor Framework
- `/biomaterials-tissue-healing/` — Safe-by-Design Hydrogels, Biomimetic Scaffolds & Wound Healing Phase Gateways
- `/biosensing-scaffolds/` — Biocompatible Wound-Monitoring Biosensors, Micro-Encapsulation & ISO 10993-12 Leachables
- `/nanocarrier-safety-directory/` — Regulatory & Clinical Nanocarrier Safety Reference Directory (Liposomes, LNPs, CARPA Prophylaxis, EMA/FDA Guidances)
- `/safe-by-design-stage-gate/` — 5-Gate Safe-by-Design Preclinical Translation Roadmap & Remediation Paths
- `/methodology/` — BET Surface-Area Dose Equations, LAL/rFC/MAT Spike-Recovery Rules & Zero-Telemetry CSP Posture
- `/about/` — Independent Editorial Charter, Contact (`editorial@thm-esatt.net`), and Consolidated Historical Archive Disclosures

## Key Quantitative Thresholds
- Delivered BET Surface-Area Dose: `Mass (ug/mL) * BET SSA (m2/g) * 0.01 = cm2/mL` (Macrophage surface-area overload threshold: `10.0 cm2/mL`).
- ISO 29701 Spike Recovery Window: `50% to 150%` Positive Product Control (PPC) recovery across serial dilutions.
- Hemolysis Safety Ceiling: `< 5%` per ASTM F756 / ISO 10993-4.
- Complement CARPA Watch: `< 2x` vehicle baseline SC5b-9 / C3a in human plasma per NCL ITA-5.1.